| Date | Tm | Player | Transaction | Notes | 40-man | 25-man |
| 20-Mar | Bos | Timlin, Mike | - Injured |
| 39 | 25 |
| 26-Mar | LAD | Abreu, Tony | - Injured |
| 39 | 24 |
| 27-Mar | Bos | Moss, Brandon | - Optioned to minors |
| 39 | 24 |
| 27-Mar | Was | Cordero, Chad | - Injured |
| 37 | 24 |
| 28-Mar | Bos | Timlin, Mike | - Placed on 15-day DL | from inactive list | 39 | 24 |
| 28-Mar | Bos | Wakefield, Tim | + Activated from inactive list |
| 39 | 25 |
| 29-Mar | Oak | Braden, Dallas | - Optioned to minors |
| 40 | 24 |
| 29-Mar | Oak | Gonzalez, Carlos | - Optioned to minors | from inactive list | 40 | 24 |
| 29-Mar | Oak | Fiorentino, Jeff | - Optioned to minors |
| 40 | 23 |
| 29-Mar | Oak | Eveland, Dana | + Activated from inactive list |
| 40 | 24 |
| 29-Mar | Oak | Duchscherer, Justin | + Activated from inactive list |
| 40 | 25 |
| 30-Mar | Atl | Hampton, Mike | - Injured |
| 39 | 24 |
| 31-Mar | Mil | Cameron, Mike | - Placed on restricted list | 25 games | 38 | 25 |
| 31-Mar | Was | Dukes, Elijah | - Placed on 15-day DL |
| 37 | 23 |
| 31-Mar | Was | Schroder, Chris | + Recalled from minors |
| 37 | 24 |
| 1-Apr | KC | Olivo, Miguel | - Suspended | 4 games | 39 | 24 |
| 1-Apr | LAD | Repko, Jason | - Optioned to minors |
| 39 | 23 |
| 1-Apr | LAD | Abreu, Tony | - Placed on 15-day DL |
| 39 | 23 |
| 1-Apr | LAD | Hull, Eric | - Waived |
| 38 | 23 |
| 1-Apr | LAD | Chavez, Angel | + Purchased from minors |
| 39 | 24 |
| 1-Apr | LAD | DeWitt, Blake | + Purchased from minors |
| 40 | 25 |
| 2-Apr | NYM | Wise, Matt | - Injured |
| 36 | 24 |
| 2-Apr | NYM | Martinez, Pedro | - Placed on 15-day DL |
| 36 | 23 |
| 2-Apr | NYM | Figueroa, Nelson | + Purchased from minors |
| 37 | 24 |
| 2-Apr | Phi | Helms, Wes | - Waived |
| 34 | 24 |
| 2-Apr | Phi | Seanez, Rudy | + Signed |
| 35 | 25 |
| 2-Apr | Sea | Putz, JJ | - Placed on 15-day DL |
| 39 | 24 |
| 2-Apr | Sea | Corcoran, Roy | + Purchased from minors |
| 40 | 25 |
| 3-Apr | LAD | Chavez, Angel | - Waived |
| 39 | 24 |
| 3-Apr | LAD | Park, Chan Ho | + Purchased from minors |
| 40 | 25 |
| 3-Apr | Oak | Harden, Rich | - Injured |
| 40 | 24 |
| 3-Apr | Was | Young, Dmitri | - Injured |
| 37 | 24 |
| 4-Apr | Atl | Hampton, Mike | - Placed on 15-day DL |
| 39 | 24 |
| 4-Apr | Atl | Reyes, Jo-Jo | + Recalled from minors |
| 39 | 25 |
| 4-Apr | Col | Vizcaino, Luis | - Injured |
| 38 | 24 |
| 4-Apr | Mil | Gwynn Jr, Tony | - Injured |
| 38 | 24 |
| 4-Apr | Min | Slowey, Kevin | - Injured |
| 38 | 24 |
| 4-Apr | NYY | Cabrera, Melky | - Suspended | 2 games | 39 | 24 |
| 4-Apr | Pit | Wilson, Jack | - Injured |
| 39 | 24 |
| 4-Apr | TB | Gomes, Jonny | - Suspended | 1 game | 40 | 24 |
| 5-Apr | Det | Raburn, Ryan | - Optioned to minors |
| 36 | 24 |
| 5-Apr | Det | Beltran, Francis | + Purchased from minors |
| 37 | 25 |
| 5-Apr | KC | Tupman, Matt | - Optioned to minors |
| 39 | 23 |
| 5-Apr | KC | Nomo, Hideo | + Purchased from minors |
| 40 | 24 |
| 5-Apr | KC | Olivo, Miguel | + Reinstated |
| 40 | 25 |
| 5-Apr | LAA | Thompson, Rich | - Optioned to minors |
| 38 | 24 |
| 5-Apr | LAA | Shields, Scot | + Activated from 15-day DL |
| 38 | 25 |
| 5-Apr | Min | Cuddyer, Michael | - Placed on 15-day DL |
| 38 | 23 |
| 5-Apr | Min | Span, Denard | + Recalled from minors |
| 38 | 24 |
| 5-Apr | NYY | Albaladejo, Jonathan | - Optioned to minors |
| 39 | 23 |
| 5-Apr | NYY | Pettitte, Andy | + Activated from 15-day DL |
| 39 | 24 |
| 5-Apr | Oak | Duchscherer, Justin | - Injured |
| 40 | 23 |
| 5-Apr | Phi | Lahey, Tim | - Returned via Rule V | to Minnesota | 34 | 24 |
| 5-Apr | Phi | Lidge, Brad | + Activated from 15-day DL |
| 34 | 25 |
| 5-Apr | SD | Gerut, Jody | - Optioned to minors |
| 40 | 24 |
| 5-Apr | SD | Edmonds, Jim | + Activated from 15-day DL |
| 40 | 25 |
| 5-Apr | StL | Springer, Russ | - Placed on 15-day DL |
| 40 | 24 |
| 5-Apr | StL | Jimenez, Kelvin | + Recalled from minors |
| 40 | 25 |
| 5-Apr | TB | Navarro, Dioner | - Placed on 15-day DL |
| 40 | 24 |
| 5-Apr | TB | Salas, Juan | - Placed on restricted list |
| 39 | 24 |
| 5-Apr | TB | Difelice, Mike | + Purchased from minors |
| 40 | 25 |
| 5-Apr | TB | Gomes, Jonny | + Reinstated |
| 40 | 25 |
| 5-Apr | Tex | Guardado, Eddie | - Injured |
| 40 | 24 |
| 5-Apr | Was | Cordero, Chad | - Placed on 15-day DL |
| 37 | 23 |
| 5-Apr | Was | Lannan, John | + Recalled from minors |
| 37 | 24 |
| 6-Apr | Atl | Reyes, Jo-Jo | - Optioned to minors |
| 39 | 24 |
| 6-Apr | Atl | Smoltz, John | + Activated from 15-day DL |
| 39 | 25 |
| 6-Apr | Bos | Snyder, Kyle | - Waived |
| 38 | 24 |
| 6-Apr | Bos | Beckett, Josh | + Activated from 15-day DL |
| 38 | 25 |
| 6-Apr | Col | Vizcaino, Luis | - Placed on 15-day DL |
| 38 | 24 |
| 6-Apr | Col | Speier, Ryan | + Recalled from minors |
| 38 | 25 |
| 6-Apr | Fla | Wood, Jason | - Waived |
| 39 | 24 |
| 6-Apr | Fla | Helms, Wes | + Acquired from | waivers | 40 | 25 |
| 6-Apr | Hou | Wigginton, Ty | - Injured |
| 35 | 24 |
| 6-Apr | NYY | Duncan, Shelly | - Suspended | 2 games | 39 | 23 |
| 6-Apr | NYY | Cabrera, Melky | + Reinstated |
| 39 | 24 |
| 6-Apr | Pit | Wilson, Jack | - Placed on 15-day DL |
| 39 | 24 |
| 6-Apr | Pit | Bixler, Brian | + Recalled from minors |
| 39 | 25 |
| 7-Apr | Ari | Coutlangus, Jon | + Acquired from | waivers and optioned | 40 | 25 |
| 7-Apr | Atl | Soriano, Rafael | - Injured |
| 39 | 24 |
| 7-Apr | Fla | VandenHurk, Rick | - Optioned to minors |
| 40 | 24 |
| 7-Apr | Fla | Garcia, Harvey | - Transferred to 60-day DL |
| 39 | 24 |
| 7-Apr | Fla | Badenhop, Burke | + Purchased from minors |
| 40 | 25 |
| 7-Apr | TB | Floyd, Cliff | - Injured |
| 40 | 24 |
| 8-Apr | Bos | Hull, Eric | + Acquired from | waivers and optioned | 39 | 25 |
| 8-Apr | Fla | Carroll, Brett | - Optioned to minors |
| 40 | 24 |
| 8-Apr | Fla | Hermida, Jeremy | + Activated from 15-day DL |
| 40 | 25 |
| 8-Apr | NYM | Wise, Matt | - Placed on 15-day DL |
| 37 | 24 |
| 8-Apr | NYM | Muniz, Carlos | + Recalled from minors |
| 37 | 25 |
| 8-Apr | NYY | Duncan, Shelly | + Reinstated |
| 39 | 25 |
| 8-Apr | Oak | Duchscherer, Justin | - Placed on 15-day DL |
| 40 | 23 |
| 8-Apr | Oak | Gaudin, Chad | + Activated from 15-day DL |
| 40 | 24 |
| 8-Apr | SD | Barrett, Michael | - Placed on 15-day DL |
| 40 | 24 |
| 8-Apr | SD | Morton, Colt | + Recalled from minors |
| 40 | 25 |
| 8-Apr | SF | Roberts, Dave | - Placed on 15-day DL |
| 40 | 24 |
| 8-Apr | SF | Timpner, Clay | + Recalled from minors |
| 40 | 25 |
| 8-Apr | Was | Young, Dmitri | - Placed on 15-day DL |
| 37 | 24 |
| 8-Apr | Was | Estrada, Johnny | + Activated from 15-day DL |
| 37 | 25 |
| 9-Apr | Ari | Davis, Doug | - Placed on 15-day DL |
| 40 | 24 |
| 9-Apr | Ari | Peguero, Jailen | + Recalled from minors |
| 40 | 25 |
| 9-Apr | Atl | Soriano, Rafael | - Placed on 15-day DL |
| 39 | 24 |
| 9-Apr | Atl | James, Chuck | + Activated from 15-day DL |
| 39 | 25 |
| 9-Apr | ChC | Pignatiello, Carmen | - Optioned to minors |
| 39 | 24 |
| 9-Apr | ChC | Marshall, Sean | + Recalled from minors |
| 39 | 25 |
| 9-Apr | Mil | Gwynn Jr, Tony | - Placed on 15-day DL |
| 38 | 24 |
| 9-Apr | Mil | Iribarren, Hernan | + Recalled from minors |
| 38 | 25 |
| 9-Apr | NYY | Duncan, Shelly | - Optioned to minors |
| 39 | 24 |
| 9-Apr | NYY | Gonzalez, Alberto | + Recalled from minors |
| 39 | 25 |
| 9-Apr | Oak | Johnson, Dan | - Waived |
| 39 | 23 |
| 9-Apr | Oak | Smith, Greg | + Purchased from minors |
| 40 | 24 |
| 9-Apr | Sea | Bedard, Erik | - Injured |
| 40 | 24 |
| 9-Apr | TB | Garza, Matt | - Placed on 15-day DL |
| 40 | 23 |
| 9-Apr | TB | Floyd, Cliff | - Placed on 15-day DL |
| 40 | 23 |
| 9-Apr | TB | Ruggiano, Justin | + Recalled from minors |
| 40 | 24 |
| 9-Apr | TB | Ryu, Jae Kuk | + Recalled from minors |
| 40 | 25 |
| 9-Apr | Tor | Wells, Randy | - Returned via Rule V |
| 39 | 24 |
| 9-Apr | Tor | Carlson, Jesse | + Purchased from minors |
| 40 | 25 |
| 10-Apr | Atl | James, Chuck | - Optioned to minors |
| 39 | 24 |
| 10-Apr | Atl | Campillo, Jorge | + Purchased from minors |
| 40 | 25 |
| 10-Apr | Bos | Cora, Alex | - Injured |
| 39 | 24 |
| 10-Apr | Bos | Lowell, Mike | - Placed on 15-day DL |
| 39 | 23 |
| 10-Apr | Bos | Lowrie, Jed | + Purchased from minors |
| 40 | 24 |
| 10-Apr | Oak | Harden, Rich | - Placed on 15-day DL |
| 40 | 24 |
| 10-Apr | Oak | Devine, Joey | + Recalled from minors |
| 40 | 25 |
| 10-Apr | TB | Aybar, Willie | - Injured |
| 40 | 24 |
| 11-Apr | Bal | Moore, Scott | - Optioned to minors |
| 38 | 24 |
| 11-Apr | Bal | Johnson, Jim | + Recalled from minors |
| 38 | 25 |
| 11-Apr | Bos | Corey, Bryan | - Waived |
| 39 | 23 |
| 11-Apr | Bos | Timlin, Mike | + Activated from 15-day DL |
| 39 | 24 |
| 11-Apr | Det | Bazardo, Yorman | - Waived |
| 36 | 24 |
| 11-Apr | Det | Rapada, Clay | + Recalled from minors |
| 36 | 25 |
| 11-Apr | Fla | Hoover, Paul | - Optioned to minors |
| 40 | 24 |
| 11-Apr | Fla | Rabelo, Mike | + Activated from 15-day DL |
| 40 | 25 |
| 11-Apr | Oak | Foulke, Keith | - Injured |
| 40 | 24 |
| 11-Apr | Sea | Jimerson, Charlton | - Waived |
| 39 | 23 |
| 11-Apr | Sea | Norton, Greg | + Purchased from minors |
| 40 | 24 |
| 12-Apr | Atl | Moylan, Peter | - Injured |
| 40 | 24 |
| 12-Apr | Det | Willis, Dontrelle | - Placed on 15-day DL |
| 36 | 24 |
| 12-Apr | Det | Raburn, Ryan | + Recalled from minors |
| 36 | 25 |
| 12-Apr | Hou | Wigginton, Ty | - Placed on 15-day DL |
| 35 | 24 |
| 12-Apr | Hou | Newhan, David | + Purchased from minors |
| 36 | 25 |
| 12-Apr | Min | Slowey, Kevin | - Placed on 15-day DL |
| 38 | 24 |
| 12-Apr | SF | Timpner, Clay | - Optioned to minors |
| 40 | 24 |
| 12-Apr | SF | Bowker, John | + Recalled from minors |
| 40 | 25 |
| 12-Apr | TB | Aybar, Willie | - Placed on 15-day DL |
| 40 | 24 |
| 12-Apr | TB | Orvella, Chad | - Transferred to 60-day DL |
| 39 | 24 |
| 12-Apr | TB | Longoria, Evan | + Purchased from minors |
| 40 | 25 |
| 12-Apr | Tex | Guardado, Eddie | - Placed on 15-day DL |
| 40 | 24 |
| 12-Apr | Tex | Mendoza, Luis | + Recalled from minors |
| 40 | 25 |
| 12-Apr | Tor | Coats, Buck | - Optioned to minors |
| 40 | 24 |
| 12-Apr | Tor | Inglett, Joe | + Recalled from minors |
| 40 | 25 |
| 12-Apr | Was | Bergmann, Jason | - Optioned to minors |
| 37 | 24 |
| 12-Apr | Was | Cordero, Chad | + Activated from 15-day DL |
| 37 | 25 |
| 13-Apr | Min | Liriano, Francisco | + Recalled from minors |
| 38 | 25 |
| 13-Apr | Phi | Victorino, Shane | - Placed on 15-day DL |
| 34 | 24 |
| 13-Apr | Phi | Snelling, Chris | + Purchased from minors |
| 35 | 25 |
| 13-Apr | StL | Jimenez, Kelvin | - Optioned to minors |
| 40 | 24 |
| 13-Apr | StL | Pineiro, Joel | + Activated from 15-day DL |
| 40 | 25 |
| 13-Apr | TB | Ryu, Jae Kuk | - Optioned to minors |
| 40 | 24 |
| 13-Apr | TB | Niemann, Jeff | + Recalled from minors |
| 40 | 25 |
| 13-Apr | Tex | Fukumori, Kazuo | - Optioned to minors |
| 40 | 24 |
| 13-Apr | Tex | Feldman, Scott | + Recalled from minors |
| 40 | 25 |
| 13-Apr | Tor | League, Brandon | - Optioned to minors |
| 40 | 24 |
| 13-Apr | Tor | Ryan, BJ | + Activated from 15-day DL |
| 40 | 25 |
| 13-Apr | Was | Flores, Jesus | - Optioned to minors |
| 37 | 24 |
| 13-Apr | Was | Pena, Wily Mo | + Activated from 15-day DL |
| 37 | 25 |
| 14-Apr | Ari | Peguero, Jailen | - Optioned to minors |
| 40 | 24 |
| 14-Apr | Ari | Johnson, Randy | + Activated from 15-day DL |
| 40 | 25 |
| 14-Apr | Atl | Glavine, Tom | - Injured |
| 40 | 23 |
| 14-Apr | Hou | Newhan, David | - Waived |
| 35 | 24 |
| 14-Apr | Hou | Quintero, Humberto | + Purchased from minors |
| 36 | 25 |
| 14-Apr | LAA | Kendrick, Howie | - Injured |
| 38 | 24 |
| 14-Apr | NYY | Betemit, Wilson | - Placed on 15-day DL |
| 39 | 24 |
| 14-Apr | NYY | Chamberlain, Joba | - Placed on bereavement list |
| 39 | 23 |
| 14-Apr | NYY | Moeller, Chad | + Purchased from minors |
| 40 | 24 |
| 14-Apr | NYY | Albaladejo, Jonathan | + Recalled from minors |
| 40 | 25 |
| 14-Apr | Oak | Hernandez, Fernando | - Returned via Rule V |
| 39 | 23 |
| 14-Apr | Oak | Saarloos, Kirk | + Purchased from minors |
| 40 | 24 |
| 14-Apr | Sea | O'Flaherty, Eric | - Optioned to minors |
| 40 | 23 |
| 14-Apr | Sea | Morse, Mike | - Placed on 15-day DL |
| 40 | 22 |
| 14-Apr | Sea | Huber, Jon | - Waived |
| 39 | 22 |
| 14-Apr | Sea | Rhodes, Arthur | + Purchased from minors |
| 40 | 23 |
| 14-Apr | Sea | Dickey, RA | + Recalled from minors |
| 40 | 24 |
| 14-Apr | Tex | Feldman, Scott | - Optioned to minors |
| 40 | 24 |
| 14-Apr | Tex | Littleton, Wes | + Recalled from minors |
| 40 | 25 |
| 14-Apr | Was | LoDuca, Paul | - Injured |
| 37 | 24 |
| 15-Apr | Atl | Moylan, Peter | - Placed on 15-day DL |
| 40 | 23 |
| 15-Apr | Atl | Carlyle, Buddy | + Recalled from minors |
| 40 | 24 |
| 15-Apr | ChW | Thome, Jim | - Suspended | 1 game | 39 | 24 |
| 15-Apr | Cle | Borowski, Joe | - Placed on 15-day DL |
| 39 | 24 |
| 15-Apr | Cle | Mastny, Tom | + Recalled from minors |
| 39 | 25 |
| 15-Apr | Min | Everett, Adam | - Injured |
| 38 | 24 |
| 15-Apr | NYM | Muniz, Carlos | - Optioned to minors |
| 37 | 24 |
| 15-Apr | NYM | Sanchez, Duaner | + Activated from 15-day DL |
| 37 | 25 |
| 15-Apr | SD | Gonzalez, Enrique | - Outrighted to minors |
| 39 | 24 |
| 15-Apr | SD | Cameron, Kevin | + Recalled from minors |
| 39 | 25 |
| 15-Apr | Sea | Bedard, Erik | - Placed on 15-day DL |
| 40 | 24 |
| 15-Apr | TB | Reyes, Al | - Injured |
| 40 | 24 |
| 16-Apr | Bos | Cora, Alex | - Placed on 15-day DL |
| 39 | 24 |
| 16-Apr | Bos | Thurston, Joe | + Purchased from minors |
| 40 | 25 |
| 16-Apr | ChC | Soriano, Alfonso | - Placed on 15-day DL |
| 39 | 24 |
| 16-Apr | ChC | Patterson, Eric | + Recalled from minors |
| 39 | 25 |
| 16-Apr | ChW | Thome, Jim | + Reinstated |
| 39 | 25 |
| 16-Apr | Det | Beltran, Francis | - Waived |
| 35 | 24 |
| 16-Apr | Det | Galarraga, Armando | + Recalled from minors |
| 35 | 25 |
| 16-Apr | KC | Bale, John | - Injured |
| 40 | 24 |
| 16-Apr | LAA | Willits, Reggie | - Optioned to minors |
| 38 | 23 |
| 16-Apr | LAA | Serrano, Alex | + Purchased from minors |
| 39 | 24 |
| 16-Apr | LAD | Troncoso, Ramon | - Optioned to minors |
| 40 | 24 |
| 16-Apr | LAD | Garciaparra, Nomar | + Activated from 15-day DL |
| 40 | 25 |
| 16-Apr | Sea | Morrow, Brandon | + Recalled from minors |
| 40 | 25 |
| 16-Apr | SF | Yabu, Keiichi | - Optioned to minors |
| 40 | 24 |
| 16-Apr | SF | Chulk, Vinny | + Activated from 15-day DL |
| 40 | 25 |
| 16-Apr | TB | Reyes, Al | - Placed on 15-day DL |
| 40 | 24 |
| 16-Apr | TB | Birkins, Kurt | + Activated from 15-day DL |
| 40 | 25 |
| 16-Apr | Was | Schroder, Chris | - Optioned to minors |
| 37 | 23 |
| 16-Apr | Was | Nieves, Wil | + Purchased from minors |
| 37 | 24 |
| 17-Apr | KC | Bale, John | - Placed on 15-day DL |
| 40 | 24 |
| 17-Apr | KC | Peralta, Johnny | + Recalled from minors |
| 40 | 25 |
| 17-Apr | Mil | Iribarren, Hernan | - Optioned to minors |
| 38 | 24 |
| 17-Apr | Mil | Stetter, Mitch | + Recalled from minors |
| 38 | 25 |
| 17-Apr | Phi | Snelling, Chris | - Injured |
| 35 | 24 |
| 17-Apr | Tex | Byrd, Marlon | - Placed on 15-day DL |
| 40 | 24 |
| 17-Apr | Tex | Diamond, Thomas | - Placed on 60-day DL |
| 39 | 24 |
| 17-Apr | Tex | Duran, German | + Purchased from minors |
| 40 | 25 |
| 17-Apr | Tor | Wolfe, Brian | - Placed on 15-day DL |
| 40 | 24 |
| 17-Apr | Tor | Purcey, David | + Recalled from minors |
| 40 | 25 |
| 18-Apr | Atl | Glavine, Tom | - Placed on 15-day DL |
| 40 | 24 |
| 18-Apr | Atl | James, Chuck | + Recalled from minors |
| 40 | 25 |
| 18-Apr | ChW | Owens, Jerry | + Activated from 15-day DL | and optioned | 39 | 25 |
| 18-Apr | Fla | Gardner, Lee | - Placed on 15-day DL |
| 40 | 24 |
| 18-Apr | Fla | Mitre, Sergio | - Transferred to 60-day DL |
| 39 | 24 |
| 18-Apr | Fla | Waechter, Doug | + Purchased from minors |
| 40 | 25 |
| 18-Apr | Hou | Quintero, Humberto | - Waived |
| 35 | 24 |
| 18-Apr | Hou | Matsui, Kaz | + Activated from 15-day DL |
| 35 | 25 |
| 18-Apr | LAA | Kendrick, Howie | - Placed on 15-day DL |
| 39 | 24 |
| 18-Apr | LAA | Rodriguez, Sean | + Recalled from minors |
| 39 | 25 |
| 18-Apr | NYY | Albaladejo, Jonathan | - Optioned to minors |
| 40 | 24 |
| 18-Apr | NYY | Ramirez, Edwar | + Recalled from minors |
| 40 | 25 |
| 18-Apr | TB | Medlock, Calvin | - Waived |
| 39 | 25 |
| 18-Apr | TB | Johnson, Dan | + Acquired from | waivers and optioned | 40 | 25 |
| 19-Apr | ChC | Patterson, Eric | - Optioned to minors |
| 39 | 24 |
| 19-Apr | ChC | Murton, Matt | + Recalled from minors |
| 39 | 25 |
| 19-Apr | Min | Everett, Adam | - Placed on 15-day DL |
| 38 | 24 |
| 19-Apr | Min | Buscher, Brian | + Recalled from minors |
| 38 | 25 |
| 19-Apr | NYY | Ramirez, Edwar | - Optioned to minors |
| 40 | 24 |
| 19-Apr | NYY | Chamberlain, Joba | + Activated from bereave list |
| 40 | 25 |
| 19-Apr | Oak | Buck, Travis | - Injured |
| 40 | 23 |
| 19-Apr | SF | Threets, Erik | - Placed on 15-day DL |
| 40 | 24 |
| 19-Apr | SF | Yabu, Keiichi | + Recalled from minors |
| 40 | 25 |
| 19-Apr | Tor | Purcey, David | - Optioned to minors |
| 40 | 24 |
| 19-Apr | Tor | Banks, Josh | - Waived |
| 39 | 24 |
| 19-Apr | Tor | Camp, Shawn | + Purchased from minors |
| 40 | 25 |
| 19-Apr | Was | LoDuca, Paul | - Placed on 15-day DL |
| 37 | 24 |
| 19-Apr | Was | Hill, Shawn | + Activated from 15-day DL |
| 37 | 25 |
| 20-Apr | Cin | Hopper, Norris | - Injured |
| 40 | 24 |
| 20-Apr | Cin | Weathers, David | - Injured |
| 40 | 23 |
| 20-Apr | Cle | Westbrook, Jake | - Injured |
| 39 | 24 |
| 20-Apr | Hou | Rodriguez, Wandy | - Placed on 15-day DL |
| 35 | 24 |
| 20-Apr | Hou | Byrdak, Tim | + Purchased from minors |
| 36 | 25 |
| 20-Apr | KC | Nomo, Hideo | - Waived |
| 39 | 24 |
| 20-Apr | KC | Hochevar, Luke | + Recalled from minors |
| 39 | 25 |
| 20-Apr | LAA | Serrano, Alex | - Optioned to minors |
| 39 | 24 |
| 20-Apr | LAA | Bootcheck, Chris | + Activated from 15-day DL |
| 39 | 25 |
| 20-Apr | Mil | Dillon, Joe | - Optioned to minors |
| 38 | 24 |
| 20-Apr | Mil | Gallardo, Yovani | + Activated from 15-day DL |
| 38 | 25 |
| 20-Apr | Phi | Rollins, Jimmy | - Placed on 15-day DL |
| 35 | 23 |
| 20-Apr | Phi | Snelling, Chris | - Placed on 15-day DL |
| 35 | 23 |
| 20-Apr | Phi | Harman, Brad | + Recalled from minors |
| 35 | 24 |
| 20-Apr | Phi | Bohn, TJ | + Recalled from minors |
| 35 | 25 |
| 20-Apr | SF | Davis, Rajai | - Waived |
| 39 | 24 |
| 20-Apr | SF | Burriss, Emmanuel | + Purchased from minors |
| 40 | 25 |
| 20-Apr | TB | Niemann, Jeff | - Optioned to minors |
| 40 | 24 |
| 20-Apr | TB | Johnson, Dan | + Added to 25-man roster |
| 40 | 25 |
| 20-Apr | Tor | Thomas, Frank | - Released |
| 39 | 24 |
| 20-Apr | Tor | Diaz, Robinson | + Recalled from minors |
| 39 | 25 |
| 21-Apr | Cin | Weathers, David | - Placed on 15-day DL |
| 40 | 23 |
| 21-Apr | Cin | Castro, Juan | - Waived |
| 39 | 22 |
| 21-Apr | Cin | Belisle, Matt | + Activated from 15-day DL |
| 39 | 23 |
| 21-Apr | Cin | Hairston Jr, Jerry | + Purchased from minors |
| 40 | 24 |
| 21-Apr | Det | Tata, Jordan | + Activated from 15-day DL | and optioned | 35 | 25 |
| 21-Apr | LAA | Bulger, Jason | - Optioned to minors |
| 39 | 24 |
| 21-Apr | LAA | Morales, Kendry | + Recalled from minors |
| 39 | 25 |
| 21-Apr | Oak | Foulke, Keith | - Placed on 15-day DL |
| 40 | 23 |
| 21-Apr | StL | Washington, Rico | - Optioned to minors |
| 40 | 24 |
| 21-Apr | StL | Springer, Russ | + Activated from 15-day DL |
| 40 | 25 |
| 22-Apr | Bos | Thurston, Joe | - Waived |
| 39 | 24 |
| 22-Apr | Bos | Pauley, David | + Recalled from minors |
| 39 | 25 |
| 22-Apr | Cle | Westbrook, Jake | - Placed on 15-day DL |
| 39 | 24 |
| 22-Apr | Cle | Francisco, Ben | + Recalled from minors |
| 39 | 25 |
| 22-Apr | Col | Bowie, Micah | - Placed on 15-day DL |
| 39 | 24 |
| 22-Apr | Col | Newman, Josh | + Recalled from minors |
| 39 | 25 |
| 22-Apr | Oak | Braden, Dallas | + Recalled from minors |
| 40 | 24 |
| 22-Apr | Sea | Dickey, RA | - Optioned to minors |
| 40 | 24 |
| 22-Apr | Sea | Putz, JJ | + Activated from 15-day DL |
| 40 | 25 |
| 22-Apr | TB | Difelice, Mike | - Waived |
| 39 | 24 |
| 22-Apr | TB | Navarro, Dioner | + Activated from 15-day DL |
| 39 | 25 |
| 22-Apr | Tex | Gabbard, Kason | - Injured |
| 40 | 24 |
| 22-Apr | Tex | Nippert, Dustin | - Placed on 15-day DL |
| 40 | 23 |
| 22-Apr | Tex | Francisco, Frank | + Recalled from minors |
| 40 | 24 |
| 23-Apr | Bos | Pauley, David | - Optioned to minors |
| 39 | 24 |
| 23-Apr | Bos | Hansen, Craig | + Recalled from minors |
| 39 | 25 |
| 23-Apr | Cin | Hopper, Norris | - Placed on 15-day DL |
| 40 | 24 |
| 23-Apr | Cin | Ross, David | + Activated from 15-day DL |
| 40 | 25 |
| 23-Apr | Det | Thomas, Clete | - Optioned to minors |
| 35 | 24 |
| 23-Apr | Det | Granderson, Curtis | + Activated from 15-day DL |
| 35 | 25 |
| 23-Apr | Mil | Gross, Gabe | - Traded to | TB | 37 | 24 |
| 23-Apr | Mil | Gwynn Jr, Tony | + Activated from 15-day DL |
| 37 | 25 |
| 23-Apr | NYY | Bruney, Brian | - Injured |
| 40 | 24 |
| 23-Apr | Oak | Saarloos, Kirk | - Waived |
| 39 | 23 |
| 23-Apr | Oak | Davis, Rajai | + Acquired from | waivers | 40 | 24 |
| 23-Apr | SD | Banks, Josh | + Acquired from | waivers and optioned | 40 | 25 |
| 23-Apr | StL | Thompson, Brad | - Placed on 15-day DL |
| 40 | 24 |
| 23-Apr | StL | Ryan, Brendan | + Activated from 15-day DL |
| 40 | 25 |
| 23-Apr | TB | Johnson, Dan | - Waived |
| 38 | 24 |
| 23-Apr | TB | Gross, Gabe | + Acquired from | Milwaukee | 39 | 25 |
| 24-Apr | Ari | Romero, Alex | - Optioned to minors |
| 40 | 24 |
| 24-Apr | Ari | Montero, Miguel | + Activated from 15-day DL |
| 40 | 25 |
| 24-Apr | Bos | Hansen, Craig | - Optioned to minors |
| 39 | 24 |
| 24-Apr | Bos | Masterson, Justin | + Purchased from minors |
| 40 | 25 |
| 24-Apr | Hou | Perez, Tomas | - Waived |
| 35 | 24 |
| 24-Apr | Hou | Cassel, Jack | + Recalled from minors |
| 35 | 25 |
| 24-Apr | LAD | DeWitt, Blake | - Optioned to minors |
| 40 | 24 |
| 24-Apr | LAD | Wade, Cory | + Recalled from minors |
| 40 | 25 |
| 24-Apr | NYM | Clark, Brady | - Waived |
| 36 | 24 |
| 24-Apr | NYM | Molina, Gustavo | + Purchased from minors |
| 37 | 25 |
| 24-Apr | Oak | Buck, Travis | - Placed on 15-day DL |
| 40 | 24 |
| 24-Apr | Oak | Chavez, Eric | - Transferred to 60-day DL |
| 39 | 24 |
| 24-Apr | Oak | Thomas, Frank | + Signed |
| 40 | 25 |
| 24-Apr | Tex | Mendoza, Luis | - Injured |
| 40 | 23 |
| 24-Apr | Tex | Littleton, Wes | - Optioned to minors |
| 40 | 22 |
| 24-Apr | Tex | Gabbard, Kason | - Placed on 15-day DL |
| 40 | 22 |
| 24-Apr | Tex | Fukumori, Kazuo | + Recalled from minors |
| 40 | 23 |
| 24-Apr | Tex | Feldman, Scott | + Recalled from minors |
| 40 | 24 |
| 24-Apr | Was | King, Ray | - Outrighted to minors |
| 36 | 24 |
| 24-Apr | Was | O'Connor, Mike | + Purchased from minors |
| 37 | 25 |
| 25-Apr | Atl | James, Chuck | - Optioned to minors |
| 40 | 24 |
| 25-Apr | Atl | Stockman, Phil | + Recalled from minors |
| 40 | 25 |
| 25-Apr | Bal | Loewen, Adam | - Placed on 15-day DL |
| 38 | 24 |
| 25-Apr | Bal | Torres, Eider | + Purchased from minors |
| 39 | 25 |
| 25-Apr | Bos | Masterson, Justin | - Optioned to minors |
| 40 | 24 |
| 25-Apr | Bos | Martinez, Edgar | - Waived |
| 39 | 24 |
| 25-Apr | Bos | Corey, Bryan | + Purchased from minors |
| 40 | 25 |
| 25-Apr | LAA | Morales, Kendry | - Optioned to minors |
| 39 | 24 |
| 25-Apr | LAA | Rodriguez, Sean | - Optioned to minors |
| 39 | 23 |
| 25-Apr | LAA | Arredondo, Jose | + Recalled from minors |
| 39 | 24 |
| 25-Apr | LAA | Thompson, Rich | + Recalled from minors |
| 39 | 25 |
| 25-Apr | Min | Span, Denard | - Optioned to minors |
| 38 | 24 |
| 25-Apr | Min | Liriano, Francisco | - Optioned to minors |
| 38 | 23 |
| 25-Apr | Min | Cuddyer, Michael | + Activated from 15-day DL |
| 38 | 24 |
| 25-Apr | Min | Korecky, Bobby | + Recalled from minors |
| 38 | 25 |
| 25-Apr | NYY | Bruney, Brian | - Placed on 15-day DL |
| 40 | 24 |
| 25-Apr | NYY | Moeller, Chad | - Waived |
| 39 | 23 |
| 25-Apr | NYY | Albaladejo, Jonathan | + Recalled from minors |
| 39 | 24 |
| 25-Apr | NYY | Britton, Chris | + Recalled from minors |
| 39 | 25 |
| 25-Apr | TB | Ruggiano, Justin | - Optioned to minors |
| 39 | 24 |
| 25-Apr | TB | Garza, Matt | + Activated from 15-day DL |
| 39 | 25 |
| 25-Apr | Tex | Mendoza, Luis | - Placed on 15-day DL |
| 40 | 24 |
| 25-Apr | Tex | Melhuse, Adam | - Released |
| 39 | 23 |
| 25-Apr | Tex | Guardado, Eddie | + Activated from 15-day DL |
| 39 | 24 |
| 25-Apr | Tex | Saltalamacchia, Jarrod | + Recalled from minors |
| 39 | 25 |
| 25-Apr | Tor | Diaz, Robinson | - Optioned to minors |
| 39 | 24 |
| 25-Apr | Tor | Rolen, Scott | + Activated from 15-day DL |
| 39 | 25 |
| 26-Apr | Atl | Stockman, Phil | - Optioned to minors |
| 40 | 24 |
| 26-Apr | Atl | Schreiber, Zach | - Waived |
| 39 | 24 |
| 26-Apr | Atl | Lillibridge, Brent | + Purchased from minors |
| 40 | 25 |
| 26-Apr | Bos | Casey, Sean | - Placed on 15-day DL |
| 40 | 24 |
| 26-Apr | Bos | Moss, Brandon | + Recalled from minors |
| 40 | 25 |
| 26-Apr | Cle | Francisco, Ben | - Optioned to minors |
| 39 | 24 |
| 26-Apr | Cle | Sowers, Jeremy | + Recalled from minors |
| 39 | 25 |
| 26-Apr | Col | Nix, Jayson | - Waived |
| 38 | 24 |
| 26-Apr | Col | Capellan, Jose | + Purchased from minors |
| 39 | 25 |
| 26-Apr | LAD | Garciaparra, Nomar | - Placed on 15-day DL |
| 40 | 24 |
| 26-Apr | LAD | DeWitt, Blake | + Recalled from minors |
| 40 | 25 |
| 26-Apr | NYY | Traber, Billy | - Optioned to minors |
| 39 | 24 |
| 26-Apr | NYY | Duncan, Shelly | + Recalled from minors |
| 39 | 25 |
| 26-Apr | Oak | Dinardo, Lenny | - Optioned to minors |
| 40 | 24 |
| 26-Apr | Oak | Duchscherer, Justin | + Activated from 15-day DL |
| 40 | 25 |
| 26-Apr | Sea | Corcoran, Roy | - Optioned to minors |
| 40 | 24 |
| 26-Apr | Sea | Bedard, Erik | + Activated from 15-day DL |
| 40 | 25 |
| 26-Apr | TB | Glover, Gary | - Injured |
| 39 | 24 |
| 26-Apr | TB | Johnson, Elliot | - Optioned to minors |
| 39 | 23 |
| 26-Apr | TB | Cannizaro, Andy | + Purchased from minors |
| 40 | 24 |
| 26-Apr | Tex | Blalock, Hank | - Injured |
| 39 | 24 |
| 26-Apr | Tex | Fukumori, Kazuo | - Optioned to minors |
| 39 | 23 |
| 26-Apr | Tex | Ponson, Sidney | + Purchased from minors |
| 40 | 24 |
| 26-Apr | Tor | Inglett, Joe | - Optioned to minors |
| 39 | 24 |
| 26-Apr | Tor | Lind, Adam | + Recalled from minors |
| 39 | 25 |
| 27-Apr | Ari | Petit, Yusmeiro | - Optioned to minors |
| 40 | 24 |
| 27-Apr | Ari | Scherzer, Max | + Recalled from minors |
| 40 | 25 |
| 27-Apr | ChW | MacDougal, Mike | - Optioned to minors |
| 39 | 24 |
| 27-Apr | ChW | Wasserman, Ehren | + Recalled from minors |
| 39 | 25 |
| 27-Apr | Cle | Sowers, Jeremy | - Optioned to minors |
| 39 | 24 |
| 27-Apr | Cle | Snyder, Brad | + Recalled from minors |
| 39 | 25 |
| 27-Apr | NYY | Posada, Jorge | - Injured |
| 39 | 24 |
| 27-Apr | Pit | Morris, Matt | - Released |
| 38 | 24 |
| 27-Apr | Pit | VanBenschoten, John | + Recalled from minors |
| 38 | 25 |
| 27-Apr | SF | Correia, Kevin | - Placed on 15-day DL |
| 40 | 24 |
| 27-Apr | SF | Misch, Pat | + Recalled from minors |
| 40 | 25 |
| 28-Apr | Atl | Smoltz, John | - Injured |
| 40 | 24 |
| 28-Apr | Cin | Coffey, Todd | - Optioned to minors |
| 40 | 24 |
| 28-Apr | Cin | Bray, Bill | + Recalled from minors |
| 40 | 25 |
| 28-Apr | Cle | Snyder, Brad | - Optioned to minors |
| 39 | 24 |
| 28-Apr | Cle | Laffey, Aaron | + Recalled from minors |
| 39 | 25 |
| 28-Apr | LAA | Izturis, Maicer | - Injured |
| 39 | 24 |
| 28-Apr | LAA | Arredondo, Jose | - Optioned to minors |
| 39 | 23 |
| 28-Apr | LAA | Thompson, Rich | - Optioned to minors |
| 39 | 22 |
| 28-Apr | LAA | Wood, Brandon | + Recalled from minors |
| 39 | 23 |
| 28-Apr | LAA | Wilson, Bobby | + Recalled from minors |
| 39 | 24 |
| 28-Apr | Mil | Bush, Dave | - Optioned to minors |
| 37 | 24 |
| 28-Apr | NYY | Posada, Jorge | - Placed on 15-day DL |
| 39 | 24 |
| 28-Apr | NYY | Stewart, Chris | + Purchased from minors |
| 40 | 25 |
| 29-Apr | Atl | Smoltz, John | - Placed on 15-day DL |
| 40 | 24 |
| 29-Apr | Atl | Glavine, Tom | + Activated from 15-day DL |
| 40 | 25 |
| 29-Apr | Bal | Fahey, Brandon | - Optioned to minors |
| 39 | 24 |
| 29-Apr | Bal | Aquino, Greg | - Waived |
| 38 | 23 |
| 29-Apr | Bal | McCrory, Bob | + Recalled from minors |
| 38 | 24 |
| 29-Apr | Bal | Olsen, Garrett | + Recalled from minors |
| 38 | 25 |
| 29-Apr | Bos | Corey, Bryan | - Waived |
| 39 | 24 |
| 29-Apr | Bos | Lowell, Mike | + Activated from 15-day DL |
| 39 | 25 |
| 29-Apr | Col | Wells, Kip | - Injured |
| 39 | 24 |
| 29-Apr | LAA | Wilson, Bobby | - Optioned to minors |
| 39 | 23 |
| 29-Apr | LAA | Brown, Mark | + Purchased from minors |
| 40 | 24 |
| 29-Apr | Mil | Cameron, Mike | + Activated from restricted list |
| 38 | 25 |
| 29-Apr | Min | Bush, Dave | - Optioned to minors |
| 38 | 24 |
| 29-Apr | NYY | Britton, Chris | - Optioned to minors |
| 40 | 24 |
| 29-Apr | NYY | Ramirez, Edwar | + Recalled from minors |
| 40 | 25 |
| 29-Apr | Phi | Bohn, TJ | - Optioned to minors |
| 35 | 24 |
| 29-Apr | Phi | Robles, Oscar | + Acquired from | waivers and optioned | 36 | 24 |
| 29-Apr | Phi | Victorino, Shane | + Activated from 15-day DL |
| 36 | 25 |
| 29-Apr | SD | Zarate, Mauro | - Outrighted to minors |
| 39 | 25 |
| 29-Apr | SD | Robles, Oscar | - Waived |
| 38 | 25 |
| 29-Apr | Tex | Blalock, Hank | - Placed on 15-day DL |
| 40 | 24 |
| 29-Apr | Tex | Botts, Jason | - Waived |
| 39 | 23 |
| 29-Apr | Tex | Shelton, Chris | + Purchased from minors |
| 40 | 24 |
| 29-Apr | Tex | Boggs, Brandon | + Recalled from minors |
| 40 | 25 |
| 30-Apr | Bal | Bierd, Randor | - Injured |
| 38 | 24 |
| 30-Apr | Col | Tulowitzki, Troy | - Injured |
| 39 | 23 |
| 30-Apr | Col | Morales, Franklin | - Optioned to minors |
| 39 | 22 |
| 30-Apr | Col | Wells, Kip | - Placed on 15-day DL |
| 39 | 22 |
| 30-Apr | Col | Capellan, Jose | - Waived |
| 38 | 21 |
| 30-Apr | Col | Grilli, Jason | + Acquired from | Det | 39 | 22 |
| 30-Apr | Col | Herrera, Jonathan | + Recalled from minors |
| 39 | 23 |
| 30-Apr | Col | Quintanilla, Omar | + Recalled from minors |
| 39 | 24 |
| 30-Apr | Det | Grilli, Jason | - Traded to | Col | 34 | 24 |
| 30-Apr | Det | Cruceta, Francisco | + Activated from restricted list |
| 35 | 25 |
| 30-Apr | LAA | Wilson, Bobby | - Optioned to minors |
| 40 | 23 |
| 30-Apr | LAA | Brown, Matt | + Recalled from minors |
| 40 | 24 |
| 30-Apr | NYY | Stewart, Chris | - Optioned to minors |
| 40 | 24 |
| 30-Apr | NYY | Rodriguez, Alex | - Placed on 15-day DL |
| 40 | 23 |
| 30-Apr | NYY | Henn, Sean | + Activated from 15-day DL | and waived | 39 | 23 |
| 30-Apr | NYY | Britton, Chris | + Recalled from minors |
| 39 | 24 |
| 30-Apr | NYY | Moeller, Chad | + Signed |
| 40 | 25 |
| 30-Apr | Sea | Wilkerson, Brad | - Waived |
| 39 | 24 |
| 30-Apr | Sea | Norton, Greg | - Waived |
| 38 | 23 |
| 30-Apr | Sea | Clement, Jeff | + Recalled from minors |
| 38 | 24 |
| 30-Apr | Sea | Balentien, Wladimir | + Recalled from minors |
| 38 | 25 |
| 30-Apr | Was | Cordero, Chad | - Injured |
| 37 | 24 |
